University of Florida Saving Citrus: Using genetics to boost resistance to citrus greening

Drive through any orange grove in Florida and you are likely to see the blotched yellow stain of disease attacking the very heart of the Sunshine State. Huanglongbing (known as “HLB” or “citrus greening”) has infected citrus farms in every Florida county and caused billions of dollars in damage to one of the state’s primary crops. 

Citrus greening is caused by a bacteria that hitches a ride in a tiny insect, the Asian citrus psyllid. Once infested, trees produce undesirable fruit and slowly decline from a combination of deteriorating roots and tree canopy dieback. There is no known cure for citrus greening. 

At University of Florida’s Institute of Food and Agricultural Sciences, researchers are working to save Florida’s citrus industry by applying a new gene-editing technique. Known as CRISPR, it allows scientists to speed up the changes that might naturally occur in organisms by editing segments of DNA to achieve a desired outcome, such as improved disease immunity. 

When a tree is exposed to the bacteria that causes the disease, it doesn’t recognize the threat and instead welcomes the bacteria into its cells. Scientists are seeking an “off switch” in the genome of orange trees that will close its doors to the spread of citrus greening. “Our approach to protect citrus trees against HLB is to increase the potential of citrus’ own immunity,” said Dr. Wang. With the latest advances in gene editing, the team is helping the orange trees protect themselves to ensure that Florida’s citrus industry survives and thrives once again. 

“It has been a joy to witness the explosion in genetic and genomic technology, which has allowed us to achieve accomplishments in my lifetime that we could only dream of at the beginning of my career.” 

– Fred Gmitter

Retaking The Field Volume 4 “Retaking the Field: Science Breakthroughs for Thriving Farms and a Healthier Nation” is a collaborative report from 20 FedByScience universities and the SoAR Foundation. The report highlights research projects in the five Science Breakthroughs areas identified as the most important fields to advance in agriculture by the year 2030: genomics, microbiomes, sensors, data and informatics, and transdisciplinary research. View The Issue
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